Mass Spectrometry-Based Lipidomics: Methods and Protocols by Fong-Fu Hsu (Editor)

Mass Spectrometry-Based Lipidomics: Methods and Protocols (Methods in Molecular Biology) (1st Edition)
Author: Fong-Fu Hsu (Editor)

Introduction

Mass Spectrometry-Based Lipidomics: Methods and Protocols (1st Edition), edited by Fong-Fu Hsu, is a comprehensive guide to the techniques and protocols used in lipidomics research. This book focuses on the application of mass spectrometry (MS) in identifying and quantifying lipids, providing detailed methodologies for sample preparation, MS-based analysis, and data interpretation. It covers the latest advances in lipidomics, including high-resolution MS, tandem MS, and ion-mobility spectrometry. The book is designed to help both beginners and experienced researchers understand the complexities of lipid analysis and apply these methods in biomedical, pharmaceutical, and nutritional studies.

Key Points

1. Comprehensive Coverage of Lipidomics Techniques

   • The book provides detailed protocols for various MS techniques, including electrospray ionization (ESI), matrix-assisted laser desorption/ionization (MALDI), and gas chromatography-mass spectrometry (GC-MS).

2. Sample Preparation and Optimization

   • Detailed steps for lipid extraction, purification, and sample preparation are included to ensure high-quality and reproducible results in lipidomic analysis.

3. Data Analysis and Interpretation

   • The book includes guidelines for processing complex lipidomic data, using software tools, and interpreting mass spectra for accurate lipid identification and quantification.

4. Applications in Biomedical and Pharmaceutical Research

   • Examples of lipidomic studies in disease biomarker discovery, drug development, and metabolic profiling are discussed to highlight the real-world relevance of lipidomics.

5. Advanced Techniques and Future Trends

   • The book explores cutting-edge approaches such as ion-mobility spectrometry, high-resolution MS, and targeted lipidomics to enhance analytical accuracy and specificity.

Why Read This Book

This book is essential for researchers, scientists, and graduate students in biochemistry, molecular biology, and pharmacology who want to gain expertise in lipidomics. Its clear protocols and practical insights make it an excellent resource for setting up and optimizing mass spectrometry-based lipid analysis.

Conclusion

Mass Spectrometry-Based Lipidomics: Methods and Protocols edited by Fong-Fu Hsu is a valuable guide for understanding and applying advanced lipidomics techniques. It provides a balanced combination of theoretical knowledge and hands-on protocols, making it a key reference for researchers in biomedical and pharmaceutical sciences.