Immunohistochemistry and Immunocytochemistry: Methods and Protocol

Immunohistochemistry and Immunocytochemistry: Methods and Protocols
Editor: Luis Del Valle
Quality: Black White Pakistan Print

Introduction

Immunohistochemistry and Immunocytochemistry: Methods and Protocols edited by Luis Del Valle is a comprehensive guide to the techniques and applications of immunohistochemistry (IHC) and immunocytochemistry (ICC). The book presents a collection of well-established and emerging protocols, providing detailed step-by-step instructions to ensure accuracy and reproducibility. It covers the theoretical background, sample preparation, antibody selection, staining methods, and imaging analysis. The contributors emphasize troubleshooting tips and the latest advancements in the field, making it a valuable reference for researchers and clinical professionals.

Key Points

1. Foundations of IHC and ICC

   • Explains the principles behind immunohistochemistry and immunocytochemistry, including antigen-antibody interactions and detection systems.

2. Sample Preparation and Fixation

   • Provides guidelines for proper tissue and cell sample handling, fixation techniques, and sectioning methods to preserve sample integrity.

3. Antibody Selection and Validation

   • Discusses how to choose appropriate primary and secondary antibodies, including specificity testing and controls.

4. Staining Techniques and Detection

   • Describes various staining methods (chromogenic and fluorescent), imaging systems, and signal amplification strategies.

5. Troubleshooting and Data Interpretation

   • Offers solutions to common technical issues and guidance on analyzing and interpreting results for publication-quality images.

Why Read This Book

This book is ideal for researchers, pathologists, and laboratory technicians seeking to master IHC and ICC techniques. Its detailed protocols, troubleshooting advice, and emphasis on reproducibility make it a valuable resource for both novice and experienced professionals.

Conclusion

Immunohistochemistry and Immunocytochemistry: Methods and Protocols edited by Luis Del Valle is a practical and authoritative guide to mastering immunostaining techniques. Its clear protocols, expert insights, and emphasis on reproducibility make it an essential reference for professionals in biomedical research and clinical pathology.